fitc conjugated rat anti cd169  (Bio-Rad)

Journal: Cell

Article Title: Lymph Nodes are Innervated by a Unique Population of Sensory Neurons with Immunomodulatory Potential

doi: 10.1016/j.cell.2020.11.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: All the sections with tdTomato + cells were imaged at 20x using Olympus FV3000 confocal imaging system. . Intravital two-photon microscopy Adult Nav1.8 Cre/+ ; Rosa26 LSL-tdTomato/+ animals (6–12 weeks) were given 1 μg of FITC-conjugated rat anti-CD169 antibody (BioRad) diluted in a total volume of 20 μl of PBS into the right footpad to label CD169 + subscapular macrophages inside the draining LN.

Techniques: Virus, Recombinant, DNA Library Preparation, RNAscope, Multiplex Assay, Gene Expression, Software, Microinjection

Journal: Cell

Article Title: Lymph Nodes are Innervated by a Unique Population of Sensory Neurons with Immunomodulatory Potential

doi: 10.1016/j.cell.2020.11.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti mCherry ACRIS Cat# AB0040-200, 1:500 Rabbit anti-CGRP Immunostar Cat# 24112, 1:500 Chicken anti-GFP Aves Labs Cat# GFP-1020, 1:500 Chicken anti-NF200 Aves Labs Cat# NFH, 1:500 Rabbit anti-Tyrosine Hydroxylase Millipore Cat# AB152, 1:500 Rabbit anti-βIII-Tubulin Biolegend Cat# 802001, 1:500 Alexa Fluor 647-conjugated rat anti-CD31 Biolegend Cat# 102416, 1:50 FITC–conjugated mouse anti-smooth muscle actin (aSMA) Sigma Cat# F3777-.2ML, 1:500 eFluor 660-conjugated mouse anti-smooth muscle actin (aSMA) Thermo Fisher Cat# 50-9760-82, 1:100 eFluor 660-conjugated rat anti-CD169 Thermo Fisher Cat# 50-5755-80, 1:100 Pacific Blue-conjugated rat anti-CD45 Biolegend Cat# 103126, 1:50 Alexa Fluor 488-conjugated rat anti-PNAd Thermo Fisher Cat# 53-6036-82, 1:50 FITC-conjugated rat anti-CD169 BioRad Cat# MCA947F Bacterial and Virus Strains AAV2/1.CMV.HI.eGFP-Cre.WPRE.SV40 Gift from Wilson J. M. Addgene, Cat# 105545-AAV1, titer ≥ 8E+12 vg/ml AAV2/1.CAG.Flex.tdTomato.WPRE.bGH Oh et al.

Techniques: Recombinant, Multiplex Assay, Expressing, Software